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  Page 145

  • • ...the larvae of transgenic lines Tg(lyz:hsp70-2A-EGFP) x Tg(krt4:hsp70-2A-mCherry) (A), Tg(lyz:hsp27-2A-mCherry) (B) or Tg(lyz:xbp1s-2A- EGFP) x Tg(krt4:xbp1s-2A-mCherry) (C) at 3 dpf.
    • Experiments were performed on Tg(lyz:hsp70- 2A-EGFP) (D), Tg(krt4:hsp70-2A-mCherry) (E), Tg(lyz:hsp27-2A-mCherry) (F), Tg(lyz:xbp1s-2A-EGFP) (G) or Tg(krt4:xbp1s-2A-mCherry) (H) larvae at 3 dpf.
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  Page 125

  • • ...generated: lyz-EGFP-L10a, mpeg1- EGFP-L10a, krt4-EGFP-L10a, lyz-hsp27-2A-mCherry (Zebrafish hsp27 (ATCC 10809422, accession BC097148)), lyz-hsp70-2A-EGFP (Zebrafish hsp70 (Open Biosystems Clone Id:6789418, accession BC056709)), krt4-hsp70-2A-mCherry, lyz- xbp1-2A-EGFP (Zebrafish spliced xbp1 (Open Biosystems Clone Id:3816043, accession BC044134)) and krt4-xbp1-2A-mCherry.
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  Page 170

  • • ...backbone were constructed: EGFP-MRLC (BC004994), IQGAP(CT)-2A-EGFP (IQGAP aa1547-1657; NP_003861), γTubulin-GFP (Yoo et al., 2012), Pak2a-DN-2A-mCherry (Pak2a aa1-218) (Liu et al., 2007) and Pak2a-CA-2A- mCherry (Pak2a T395E/R189G/P190A) (Liu et...
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  • •   104   Tg(mpx:mCherry-2A-Rac2D57N) treated with DMSO (left) then nocodazole (right).
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  Page 194

  • • ...projection of a neutrophil expressing γtubulin-GFP and Pak2a-DN-2A mCherry.
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  • • ...Tg(mpx:mCherry) embryo overexpressing SHIP phosphatase domain [SHIP(aa364- 826)-2A-EGFP] specifically in neutrophils.
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  Page 295

  • • ...alone (i) or with dominant negative Rac2 (mCherry-2A- Rac2 D57N) (ii) in zebrafish larvae at...
    • ...and cell area (G) in control or mCherry-2A-Rac2 D57N expressing epithelial cells were measured and...
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  Page 124

  • • ...products from a single transgene, a viral 2A peptide linker sequence was used
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  • • ...(accession XM_001922972), zebrafish EGFP-SHIP2a (accession DQ272661) and TM-SHIP1(aa364-826)-2A-EGFP [TM, plasma membrane targeting signal (Lyn kinase...
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  Page 50

  • • ...neutrophils from control or SHIP phosphatase domain [SHIP(aa364-826)-2A-EGFP] expressing neutrophils in Tg(mpx:mCherry) at 3 dpf.