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Videos, Slides, Films
Dynamic chromatin : ATP-dependent chromatin remodeling machines
- Author / Creator
- Available as
- Online
Details
- Creator
- Bradley R. Cairns
- Format
- Videos, Slides, Films
- Language
- English
- Publication
-
- London, England : Henry Stewart Talks Ltd, 2007
- Physical Details
-
- 1 streaming video file (56 min.) : digital, mono, SWF file, sound, color
- video file
- Retrieved April 10, 2024, from https://hstalks.com/bs/666/.
- Intro slide -- Chromatin packaging: the static view -- Chromatin: the dynamic view -- Building and regulating chromatin -- Loci that utilize chromatin dynamics -- Chromatin remodelers in dynamic chromatin -- Dynamic nucleosome repositioning -- ISWI and RNA Pol II are mutually exclusive -- BRM and RNA Pol II are largely coincident -- Loss of BRM function halts transcription -- Localization of BRM/PC complexes are exclusive -- Dynamic nucleosome repositioning -- The yeast remodelers: SWI/SNF and RSC -- Remodelers slide nucleosomes in-vitro -- Dynamic nucleosomes in-vivo -- Composition of SWI/SNF and ISWI -- Mechanism of chromatin remodeling: questions -- RSC and its catalytic subunit Sth1 -- The nucleosome -- The Half Nucleosome -- DNA translocation: preview -- The DNA translocation/wave model -- Mechanism of chromatin remodeling: questions -- 2-dimensional projection of the nucleosome -- Exo III mapping with RSC -- DNase I mapping of the nucleosome -- RSC envelops the nucleosome -- RSC/Sth1 induce a hypersensitive site -- Sth1 binds internally -- 2 Sth1 binding orientations -- Sth1 binds at 1 of 2 sites -- Sth1 binds nucleosome about 2 turns from dyad -- ISWI binds nucleosomal DNA at 2 locations -- RSC/Sth1 binding: summary -- Mechanism of chromatin remodeling: questions -- Sth1 pumps DNA around the nucleosome -- DNA translocases -- Evidence for translocation -- Triplex displacement model -- Triplex displacement by RSC -- Directional DNA translocation on the nucleosome -- Predicted products of Sth1/RSC translocation -- RE cleavage assay for DNA translocation -- RE exposure prediction -- RE cleavage with linkers -- Reciprocal linker prediction -- Gapped nucleosome prediction -- Gapped nucleosome data: Dra I -- Gapped nucleosome data: Fok I -- Gapped nucleosome data: Msp I -- A model for DNA translocation on nucleosomes -- Remodelers reposition and transfer nucleosomes -- RSC catalyzes nucleosome transfer -- Nucleosome transfer by DNA translocation -- Remodeler observations -- Model for factor access on nucleosome -- Chromatin remodelers in dynamic chromatin -- SWR1 complex -- Chromatin remodelers in dynamic chromatin -- Acknowledgements