1 videorecording (42 min., 16 sec.) : sound, color
video file
Retrieved April 8, 2024, from https://hstalks.com/bs/438/.
Introduction -- Macromolecular crowding and protein aggregation -- Topics to be discussed -- The principle of protein self-assembly -- Protein binding of a newly synthesized polypeptide -- Rubisco chloroplast protein -- Rubisco large subunit binding protein -- Discovery of the chaperonins -- BiP binding of the heavy chain of immunoglobulin -- Origins of the molecular chaperone concept (1) -- Origins of the molecular chaperone concept (2) -- The march of molecular chaperones -- The principle of protein self-assembly -- In vivo protein folding comparing to in vitro folding -- Protein aggregation -- A possible fate for all polypeptide -- Two key properties -- Macromolecular crowding -- Major consequences of macromolecular crowding -- The excluded volume effect -- The effect of macromolecular crowding -- Effects of crowding on reaction rate -- Predicted effects of macromolecular crowding -- Effect of crowding agents on refolding of lysozyme -- Crowding promotes the formation of amyloid fibrils -- Lysozyme avoid aggregation when folding in ER -- The solution to aggregation- in small chaperons -- The solution to aggregation- in large chaperons -- Molecular chaperones -- The molecular chaperone function