Fe-Cr-Nb-B magnetic particles and STEM cells, triggers for cancer cells apoptosis by magneto-mechanical actuation

Author / Creator

MMM 2020 (2020)

Conferences

MMM 2020 Q5: Biomedical Applications IV (2020)

Available as

Online

Summary

Recently, we have introduced a new type of magnetic particles (MPs), prepared by wet milling of superferromagnetic Fe-Cr-Nb-B precursor glassy ribbons, for cancer treatment by magneto-mechanical ac...

Recently, we have introduced a new type of magnetic particles (MPs), prepared by wet milling of superferromagnetic Fe-Cr-Nb-B precursor glassy ribbons, for cancer treatment by magneto-mechanical actuation in low magnetic fields (1÷20 Oe) [1]. In this work we studied the possibility of using adipose derived stem cells (ADSCs) for the transport of Fe-Cr-Nb-B MPs to areas with MG-63 human osteosarcoma (HOS), and the effect of MPs incorporated into ADSCs on the viability of HOS following the magneto-mechanical actuation of MPs with dimensions between 5 and 100 nm. The viability tests performed 24 hours after the magneto-mechanical actuation by MTT assay indicated values of 24÷25% for HOS and up to 10% for ADSCs. We also investigated the capacity of ADSCs to incorporate Fe-Cr-Nb-B MPs, and we found that large amounts of MPs were ingested as confirmed by a colorimetric ferrozine-based assay that quantitatively evaluates the Fe content in cells. We used a time-lapse cell imaging method to record the cellular migration of ADSCs loaded with MPs towards tumor cells in vitro. The recorded films demonstrated that MPs-loaded ADSCs were able to target osteosarcoma cells. We performed in vitro tests, where ADSCs were incubated with Fe-Cr-Nb-B MPs, then transferred onto an osteosarcoma cell culture and exposed to the rotating magnetic field. Magneto-mechanical actuation led to the destruction of ADSCs and to the release of MPs on HOS culture, which incorporated the released MPs. By magneto-mechanic actuation, the HOS viability dropped down to 21÷22%. The irregular shape of the MPs combined with their rotational motion in the rotating magnetic field produce irreversible damages on the cells membranes and organelles, and, subsequently, the cells death. Work supported by the Nucleu Programme (Project PN 19 28 01 01) and Contract No. 11PFE/16.10.2018 financed by the Romanian Ministry of Education and Research.)References: [1] H. Chiriac, E. Radu, M. Tibu, et al., Sci. Rep. 8 (2018) 11538.

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