Marijuana and cannabinoid research : methods and protocols

Available as

Online

Summary

The revolutionary discovery that the human body and brain make their own marijuana-like substances called endocannabinoids has transformed marijuana-cannabinoid research into a mainstream science i...

The revolutionary discovery that the human body and brain make their own marijuana-like substances called endocannabinoids has transformed marijuana-cannabinoid research into a mainstream science impacting our understanding of reproduction, development, and cell death. In Marijuana and Cannabinoid Research: Methods and Protocols, prominent researchers and clinicians from around the world detail the cutting-edge in vitro and in vivo methods they are using to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. Other chapters describe methods to study the role of CBrs in immune function, the morphometric cytoskeletal components of neuronal and astroglial cells after cannabinoid treatment, and the in vitro and in vivo effects of cannabinoids in animals, including humans. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and electrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Marijuana and Cannabinoid Research: Methods and Protocols will help both basic and clinical investigators unravel the specific mechanisms of the marijuana-cannabinoid actions that are of central importance in biological and therapeutic research today.

Show More