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Single molecule biophysics and poisson process approach to statistical mechanics
- Author / Creator
- Available as
- Online
- Summary
-
This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science...
This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.
Details
- Creator
- Susanta K. Sarkar
- Format
- Books
- Language
- English
- Publication
-
- San Rafael [California] (40 Oak Drive, San Rafael, CA, 94903, USA) : Morgan & Claypool Publishers, [2016]
- Bristol [England] (Temple Circus, Temple Way, Bristol BS1 6HG, UK) : IOP Publishing, [2016]
- Physical Details
-
- 1 online resource : illustrations (some color)
- data file
- ISBNs
- 9781681741161, 1681741164, 9781681742441, 1681742446, 9781681740522, 9781681740522
- OCLC
- ocn949179498
- "Version: 20160401"--Title page verso.
- "A Morgan & Claypool publication as part of IOP Concise Physics"--Title page verso.
- Includes bibliographical references.
- Dr. Susanta Sarkar℗ is an Assistant Professor and Principal Investigator of the Single Molecule Biophysics Lab at the Colorado School of Mines. He studies biomolecules and nanoparticle one by one to understand their functions and properties. He received his PhD at the University of Oregon, completed his postdoctoral study at Cornell, and worked at the National Institutes of Health before joining the School of Mines.
- Preface -- 1. Single molecule biophysics -- 1.1. Introduction -- 1.2. A brief history of single molecule study -- 1.3. Single molecule methods -- 1.4. Single molecule data analysis -- 1.5. Brief overview of thermodynamics and statistical mechanics -- 1.6. Future outlook -- 1.7. Conclusion
- 2. The total internal reflection fluorescence microscope -- 2.1. Introduction -- 2.2. Design considerations -- 2.3. Microscope frame and associated parts -- 2.4. Microscope objectives -- 2.5. Detection path and camera -- 2.6. Excitation path -- 2.7. Computer, computer software, and other computing hardware -- 2.8. Experimental protocol for slide preparation -- 2.9. Specifications of the TIRF microscope -- 2.10. Objective-type TIRFM -- 2.11. Multichannel detection and FRET -- 2.12. Circular dichroism spectroscopy and microscope enclosure -- 2.13. Acknowledgments
- 3. Poisson process approach to statistical mechanics -- 3.1. Introduction -- 3.2. Diffusion -- 3.3. Inter-conversion of the states of molecules -- 3.4. Photon statistics from lasers -- 3.5. Statistics of fluorescent centers on a surface -- 3.6. Location of stars in the sky -- 3.7. Quantifying the heterogeneity of the locations of cosmic ray sources -- 3.8. Conclusion -- 3.9. Acknowledgments
- Appendices. -- A. Building a total internal reflection fluorescence microscope: parts, vendors, and costs -- B. The Poisson process
Subjects
- Biophysics.
- Biomolecules.
- Biomolecules -- Analysis.
- Biophysics -- methods.
Medical Subject Heading
- Molecular Biology -- methods.
Medical Subject Heading