Quantitative analyses of certain enteric bacteria and bacterial extracts. 2. Discrimination of sonic extracts by interfacial densitometry of precipitin systems

Author / Creator

Glenn, William G

Available as

Physical

Summary

Bacterial extracts prepared by ultrasonication were reacted with bone marrow and broad spectrum reference (homologous) and cross-reacting (heterologous) precipitins produced in rabbits. Quantificat...

Bacterial extracts prepared by ultrasonication were reacted with bone marrow and broad spectrum reference (homologous) and cross-reacting (heterologous) precipitins produced in rabbits. Quantification of the reaction was obtained by densitometry of the antigen-antibody interface. Comparisons were made of sonicates from various starting populations all equated to the same nitrogen concentrations and of various nitrogen levels derived from five bacterial population levels prepared separately. Sources of error are probed to show under what circumstances cross-reactions would be greater magnitude than reference ones. The feasibility was shown of using quantitative densitometry of the interface combined with broadly reacting precipitins to identify bacteria on an intergenic and interspecies scale. Problems associated with the use of absorbed or monospecific precipitins are explained.

Show More