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Plant isoprenoids : methods and protocols
- Available as
- Online
- Physical
Details
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- Creator
- edited by Manuel Rodríguez-Concepción
- Format
- Books
- Language
- English
- Publication
-
- New York : Humana Press, [2014]
- Physical Details
-
- xi, 303 pages : illustrations (some color) ; 26 cm
- ISBNs
- 9781493906055, 9781493906062, 1493906054, 1493906062
- OCLC
- ocn879595656, ocn869267121
- Includes bibliographical references and index.
- Plant isoprenoids: A General Overview -- Measuring the Activity of 1-Deoxy-D-Xylulose 5-Phosphate Synthase, the First Enzyme in the MEP Pathway, in Plant Extracts -- Determination of 3-Hydroxy-3-methylglutaryl CoA Reductase Activity in Plants -- Farnesyl Diphosphate Synthase Assay -- Metabolite Profiling of Plastidial Deoxyxylulose-5-Phosphate Pathway Intermediates by Liquid Chromatography and Mass Spectrometry -- Analysis of Carotenoids and Tocopherols in Plant Matrices and Assessment of Their In Vitro Antioxidant Capacity -- Simultaneous Analyses of Oxidized and Reduced Forms of Photosynthetic Quinones by High-Performance Liquid Chromatography -- Determination of Sterol Lipids in Plant Tissues by Gas Chromatography and Q-TOF Mass Spectrometry -- Analysis of Plant Polyisoprenoids -- Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots -- Gas Chromatography Mass-Spectrometry Method for Determination of Biogenic Volatile Organic Compounds Emitted by Plants -- Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts using UPLC-qTOF Mass Spectrometry -- Isoprenoid and Metabolite Profiling of Plant Trichomes -- Sample Preparation for Single Cell Transcriptomics -- Essential Oil Glands in Citrus Fruit Peel as an Example -- Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions -- Confocal Laser Scanning Microscopy Detection of Chlorophylls and Carotenoids in Chloroplasts and Chromoplasts of Tomato Fruit -- Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS -- High Throughput Testing of Terpenoid Biosynthesis Candidate Genes Using Transient Expression in Nicotiana benthamiana -- Heterologous Stable Expression of Terpenoid Biosynthetic Genes Using the Moss Physcomitrella patens -- Quantification of Plant Resistance to Isoprenoid Biosynthesis Inhibitors -- A Flexible Protocol for Targeted Gene Co-Expression Network Analysis
Subjects
- Terpenes -- metabolism -- laboratory manuals.
Medical Subject Heading
- Plants -- metabolism -- laboratory manuals.
Medical Subject Heading
- Terpenes -- analysis -- laboratory manuals.
Medical Subject Heading
- Terpenes -- pharmacology -- laboratory manuals.
Medical Subject Heading
- Isopentenoids -- Laboratory manuals.