MULTIPLE STAINS

c generally either precipitated or turned into a straw colour.
I Marsh ('Section Cutting,' p. 85) speaks of this process as
I having given him  the best results of all double stains tried
I by him.
175. Borax-Carmine and Indigo-Carmine (Seiler's method').
- -Stain in Woodward's borax-carmine (No. 74), wash out in
HCl one part, alcohol four parts, until the sections assume a
bright rose colour (which appears in a few seconds). Wash
the acid out of the sections, and stain for six to eighteen
hours in a mixture of two drops of sulphindigotate of soda
solution with one ounce of 95 per cent. alcohol. The mixture
should be filtered before using (No. 103).
Nuclei red, formed material slightly tinged with blue.
Connective-tissue fibres deep blue, blood-vessels purplish.
" Epithelium and hair take this staining in a very curious
manner, inasmuch as the cells of different ages take different
colours, ranging from a brilliant emerald-green to purple-
violet and olive-green, thus affording a valuable means of
differentiation, especially in epitheliomas, where the so-called
pearls are brought out with general distinctness, being of a
different colour from the rest of the cells."
Permanent. An excellent process.
It is obvious that this method may be modified, in most
cases with advantage, by using Grenacher's alcoholic borax-
carmine (No. 81) instead of the aqueous solution of Wood-
ward. Heneage Gibbes uses the borax-carmine quoted under
his name, No. 75.
176. Carmine and Anilin-Blue (Duval's method2).-Stain
with carmine "in the ordinary way;" dehydrate; and stain
for a few minutes (ten minutes for a section of nerve-centres)
in an alcoholic solution of anilin-blue (ten drops of saturated
solution of anilin-blue soluble in alcohol to ten grammes of
I 'Am. Quart. Mic. Journ.,' i (1879), p. 220. ' Journ. Roy. Mic. Soc.,'
ji (1879), p. 613.
2 ' Pr6cis de technique microscopique,' 1878, p. 225.

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