METHYL-VIOLET. BISMARCK BROWN.                125
According to Whitman ('Journ. Roy. Mic. Soc.', 1. c.), this
stain is in use at Naples.
152. Anilin-Violet or Methylanilin (Weiss's methods').-
I Iodine, iodated chloride of zinc, and sulphate of indigo, are
well-known tests for amyloid substances. To these in late
years has been added anilin-violet, which       possesses the
property of staining of a more or less lively red the regions
affected with amyloid degeneration, whilst other tissues are
stained of an intense blue.
152a. Safranin.-To all of these, Weiss prefers safranin.
Safranin is perfectly soluble in alcohol, imperfectly so in water;
stains tissues in a few instants rose, nuclei more intensely
than cell substance. Both fresh and alcohol-hardened tissues
are well stained; less well those that have been hardened
with chromic acid. Preparations keep well in a saturated
solution of acetate of potash. As regards testing for amyloid
substances, the reaction is as follows: healthy tissues stain of
a fine rose colour, those affected with amyloid degeneration
of a fiery orange yellow.
The preparation employed by the author goes by the name
of " rose safranin."
153. Methyl-Violet     (Methylanilin= anilin-violet= Paris-
violet =inchiostro di Leonardi) (Capparelli's experiment 2).-
Referring to the above-mentioned dichroic reaction of anilin-
violet, Capparelli, suspecting it to be an optical and not a
chemical reaction, examined unstained sections by light
transmitted through a thin layer of anilin-violet, and found
the reaction was produced just as if they had been stained,
that is, the amyloid substance appeared red, and the healthy
tissues violet. He obtained the same effect with some other
dichroic liquids.   He then    mounted sections of affected
organs on glass stained with the seven colours of the spec-
1 'Archivio perle scienze mediche,' iii, No. 14 (1879), p. 2.
2 'Archivio perle scienze mediche,' iii, No. 21, p. 1.

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