INDIFFERENT LIQUIDS

time, but if spores of fungi begin to develop a little chloial
can be added and the fluid refiltered. The solution with
chloral is for carmine or heematoxylin objects, that with
acetate for anilin objects.
311. Corrosive Sublimate Solution (Harting's fluid ').-One
part of sublimate to from 200 to 500 of water. (For blood-
corpuscles of frog 1-400, of birds 1-300, of mammals
1-200.) "iHarting recommends this as the best preservative
for the corpuscles of the blood, nerve, muscular fibre, &c."
Pacini2 remarks that " bichloride of mercury coagulates
and precipitates the albuminous matter that exists in the
interstitial fluids of the tissues," and therefore in order to
prevent this coagulation it is well to associate with it salt for
certain preparations, or acetic acid for others.     On this
principle are prepared the following classical fluids of
Goadby and Pacini.
312. Salt, Alum, and Corrosive Sublimate (" Goadby")
(Goadby's fluid3).
Bay salt (coarse sea salt)   .    .   4 ounces.
Alum    .    .    .     .    .    .   2
Corrosive sublimate     .    .    .   2 grains.
Boiling water      .    .    .    .   I quart.
This is found to be "too strong" for most purposes, and
therefore the following is recommended for general purposes.
313. Salt, Alum, and Corrosive Sublimate (Goadby's second
fluid4).
Bay salt    .    .    .    .     .  4 ounces.
Alum        .    .    .    .    .   2
Corrosive sublimate   .    .    .   4 grains.
Water       .    .    .    .    .   2 quarts.
1 'Micro. Diet.,' Art. " Preservation," p. 640.
'Journ. de Mic.,' iv (1880). ' Journ. Roy. Mic. Soc.' (N.S.), ii (1882),
p. 702.
' Micro. Diet.,' Art. " Preservation," p. 641.
4 Ibid.

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