THE MICROTOMIST'S VADE-MECUM

CHAPTER XXXV.                               foral
INTEGUMENT AND TACTILE ORGANS OF VERTEBRATA.
506. Epithelium-cells and Gland-cells (F. E. Schultze's       nsh
methods ').-In his beautiful researches on the epidermis of    isinlg
fishes and amphibia, and on the epithelium of the intestinal quaf
canal of all classes of vertebrates, as well as on that of the
respiratory  passages of pulmonate vertebrates, Schultze        is
principally used the following reagents:                        api(
Pisces. For hardening, solutio Milleri.
For maceration, iodised serum, Miiller's solution.
Amphibia. The same.
Reptilia. The same. Maceration in saliva.                       T
Mammalia. The same. (For hardening, chromic acid of           sta
0*15 per cent. in the case of the stomach of Delphinus pho- glan
ecaena.)
507. Ear of Hedgehog (Schabl's methods ).-Inject a very     ds
thin carmine-gelatin mass; cool in ice; and treat the organ 1
for five or ten minutes with osmic acid of 1 per cent. See-      a
tions should be made at once, without further preparation.       1
To make surface-preparations, take young animals, and after
preparing the organ as above, macerate for some time in
chromic acid (002 to 004 per cent.), or in iodised serum.
Before examining, treat the preparation with alcohol con-
taining various proportions of acetic acid. Mount in acidu-
I 'Arch. Mik. Anat.,' iii (1867), p. 145.
Ibid., viii (1872), p. 296.

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