tine of anilin-blue (anilin-blue soluble only in alcohol) ; do
not wash with alcohol, clear with turpentine, mount in balsam
or dammar. Duval recommends for the solution of anilin-
blue the proportion of 10 drops of a saturated solution to 10
grammes of absolute alcohol, and an immersion of ten to
twelve minutes. A violet stain, in which different tissues
are very sharply differentiated by the predominance of the
red or the blue element; blood-vessels come out with great
distinctness, the preparations, though deeply coloured, remain
very transparent, and the definition of their elements is
remarkably good.
573. Ganglion-cells, Isolation of (Carribre's methods ').-
Carriere put sections of fresh spinal cord into the three fol-
lowing solutions:
a. Bichromate of potash 1-600.
b. Bichromate of potash 1-500.
c. Chromate of ammonia 1-600.
After ten days the sections were removed from a and b,
washed with water, and thrown into a barely-transparent
ammoniacal solution of carmine. Five days afterwards the
sections from a were found to be in a fit state of maceration
for teasing out, but in the sections from b the neuroglia was
not found sufficiently broken down, and these sections had to
be rejected. The sections in c were removed after fourteen
days into the carmine solution, and after three days therein
were found to be fit for teasing. By these means Carritre
was able to demonstrate cases of anastomoses between the
cells of the anterior cornua.
574. Cerebellum (Denissenko's method 2).-Portions of cere-
bellum are hardened for two or three weeks or more in a 2 to
5 per cent. solution of bichromate of ammonia, or in Miller's
solution. Wash in water for twenty-four hours, and pass
first into weak alcohol, then into strong. Sections are then
' Arch. Mik. Anat.,' xiv (1877), p. 126.
Ibid., p. 205.

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MYELON