THE MICROTOMIST'S VADE-MECUM

a water-bath, which takes five to ten minutes. The sections
thus fixed can be treated for days with oil of turpentine,
chloroform, alcohol, and water, without losing their adhe-
sion. Stain as desired. If any cloudiness should appear
between the sections, through the solution having been too
concentrated or laid on too thick, it may be removed by
passing a brush wetted with oil of cloves several times between
the sections."
276. White of Egg Fixing Medium (Mayer's formula1).-
A  mixture of equal volumes of filtered white of egg and
glycerin, to which are added a few drops of some antiseptic is to a
(carbolic acid). A thin layer of the mixture is spread on a       intiR
cold slide with a fine brush and the sections laid on it, and    iia
warmed for some minutes on a water-bath. As the paraffin sin
in the sections melts it carries the albumen away from them, isltt
and this is one of the advantages of the method. The sections 01 hih
may be treated with turpentine, alcohol, and aqueous or other     perI
stains without any danger of their moving.                       theakd
The function of the glycerin is merely to keep the layer iR1itati
of albumen moist.
Mayer considers that this method is an improvement on           said
those of Frenzel, Threlfall, and Schililibaum. It allows of "cleariE
the staining of sections on the slide with anilin stains, which  te
is seldom practicable with Schillibaum's method, as the col- blsam
lodion stains with most anilin stains, and does not yield up       It is
the colour to alcohol.                                            apts,
'M. T. Zool. Stat. Neapel,' iv (1883). 'Journ. Roy. Mic. Soc.' (N.S.).
iv (1884), p. 317.
most ID
Iisis1'

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