INDIFFERENT LIQUIDS                   229
For blood-corpuscles of warm-blooded animals.
318. Acetic Acid and Corrosive Sublimate (Pacini's fluid,
No. 41).
Bichloride of mercury    .    .    .    1 part.
Acetic acid   .    .     .    .    .    2
Water            .    .            . 300
"Serves best for the nuclei of animal tissues, but it swells
up the fibres and distorts the forms of the cells."
319. Salt, Sublimate, and Glycerin (Pacini's formula2).
In the place here quoted, Frey speaks of the liquids of
Pacini as differing from   those of Goadby through their
containing glycerin in lieu of alum. He gives the following
directions. Take-
Sublimate      .    .    .     .    .    1 part.
Sodium chloride      .    .    .    .    2
Glycerin (250 Beaumc)    ..    .    .   13
Water     .     .    .    .    .    . 113    ,,
Allow the mixture to remain undisturbed for at least two
months. At the end of that time, take for use 1 part, mix
with 3 parts of water, and filter. This mixture is said to be
a good preservative of all delicate tissues.
320. Acetic Acid, Sublimate, and Glycerin (Pacini's for-
mula ) :
Sublimate     .    .    .     .    .    1 part.
Acetic acid   .     .    .    .    .    2
Glycerin (25' Beaumi)    .    .    .   43
Water    .     .    .    .    .    . 115
This mixture is to be employed in the same way as the
last. It is said to destroy red blood-corpuscles, but to pre-
serve white blood-corpuscles.
321. Modifications of the foregoing Sublimate Solutions.4
-The following formule are quoted by Frey from Cornil as
being in use in the Pathological Institute of Berlin.
I ibid.
2 Frey, ' Le Microscope,' 1867, p. 233.  3 Ibid.  ' Ibid.

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