CARMINE STAINS

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I ' Trait4 Technique,' p. 97.
3 ' Practical Biology,' p. 268.

2 'Le Microscope,' p. 167.

wash out the vessels; finally inject the Prussian-blue. fluid
('low to Work, &c.,' p. 296). I have tried it for staining
sections, and small portions of tissues, in the ordinary way;
and find it answers well.
52. Ammonia-carmine (Ranvier's formula').
Distilled water .    .    .     .    .    . 100
Ammonia.        .    .    .     .         .   1
Carmine    .    .    .    .     .    .    .   1
Rub up the carmine in a mortar with a little of the water;
add the ammonia, and when the carmine is dissolved, add the
rest of the water. If there remain an excess of ammonia,
heat over a water-bath until precipitation of the carmine
begins.
53. Ammonia-carmine (Frey's formula2).
Carmine.      .     .    .    . 15 to 30 centigrammes.
Ammonia       .     .    .    . quant. suff.
Water    .    .    .    .     . 30 grammes.
Dissolve, filter, and add-
Glycerin      .    .     .    . 30 grammes.
Strong alcohol      .    .    .   8 to 12 grammes.
More glycerin may be added if desired. Ranvier quotes
this formula with approval. It is very difficult to see in what
respects it differs from Beale's formula, which he quotes with
disapproval.
54. Ammonia-carmine (Huxley's and Martin's formula3).
Carmine.      .     .    .    .   2 grammes.
Strong solution of ammonia . 4 c.c.
Distilled water     .    .    . 48 c.c.
Dissolve the carmine in the ammonia and water; leave in
an unstoppered bottle until nearly all smell of ammonia has
gone. Afterwards keep in a well-closed bottle. Dilute a
small quantity with fifteen or twenty times its bulk of water,
when required for use.

I

51